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Image Search Results
Journal: Cancers
Article Title: DNA Replication Licensing Protein MCM10 Promotes Tumor Progression and Is a Novel Prognostic Biomarker and Potential Therapeutic Target in Breast Cancer
doi: 10.3390/cancers10090282
Figure Lengend Snippet: Cancer hallmarks of stable MCM10 knockdown (KD) in MCF 7 cells. ( A , B ) relative expression of MCM10 in stable MCM10-KD MCF 7 cell lines assessed by qPCR and Western blots ( n = 3, *** p < 0.001); ( C ) apoptosis analysis by Annexin V/Propidium Iodide flow cytometry showed no difference between stable MCM10-control MCF7 cell lines and stable MCM10-KD MCF 7 cell lines ( n = 3, * p < 0.05); ( D ) following MCM10 KD, the MCM10-KD MCF7 cells showed a decrease in cell proliferation monitored for seven days using phase contrast images in Incucyte ZOOM analysis software ( n = 6); ( E ) MTT assay using stable cells showed a similar observation indicating decreased proliferation in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( F ) analysis cell cycle markers by western blot and QPCR showed a decrease in cycling D1 expression indicating impaired cell proliferation in in MCM10-KD MCF 7 cells ( n = 3, * p < 0.05); ( G ) wound healing migration assay performed by using Incucyte ZOOM showed a decrease in cell migration in MCM10-KD MCF 7 cell lines. Wound heal quantified by relative Gap size using Incucyte ZOOM software ( n = 6, * p < 0.05); ( H ) Transwell cell migration assay was used to confirm our observation and showed a similar decrease in the number of migrating cells in MCM10-KD MCF 7 cells ( n = 3, ** p < 0.01); ( I ) colony formation assay performed using soft agar showed a decrease in the number of colonies in MCM10-KD MCF 7 cells compared to the control cells. ( n = 3, * p < 0.05).
Article Snippet: Data were analyzed using the
Techniques: Expressing, Western Blot, Flow Cytometry, Software, MTT Assay, Migration, Cell Migration Assay, Colony Assay